Storage for microcuvettes kept in a vial:
Store unopened HemoCue® Glucose 201 Microcuvettes below 8 °C (46 °F) (incl. storage in a freezer). Note, microcuvettes stored in a freezer must be allowed to reach room temperature (approx. 30 minutes) before analysis. Microuvettes kept in an opened vial are stable for 30 days when stored in a refrigerator at 2–8 °C (35 – 46 °F).
After breaking the seal, the vial can be stored at room temperature for up to 3 days.
Close the lid immediately after cuvettes are removed from the vial.
Storage for individually packaged microcuvettes:
Store unopened HemoCue® Glucose 201 Microcuvettes below 8 °C (46 °F) (incl. storage in a freezer). Note, microcuvettes stored in a freezer must be allowed to reach room temperature (approx. 30 minutes) before analysis. The individually packaged microcuvettes can be stored at room temperature for up to 3 days.
Method: (1) Banauch, et. al., Z Klin Chem u Klin Biochem, 1975, 13, 101-107.
(2) Bergmeyer, Methods of Enzymatic Analysis 1, 1974, Chemie Publishers, Weinheim.
Principle: Saponin hemolyzes the erythrocytes and their contents are released. α-D-glucose is transformed to ß-D-glucose using the mutarotase enzyme. The GDH enzyme (glucose dehydrogenase), specific for ß-D-Glucose, acts as a catalyst in the oxidation of ß-D-Glucose in the presence of NAD (nicotinamide adenine dinucleotide) to form NADH. The GDH/mutarotase enzyme system ensures a molar relationship between the available glucose and the NADH produced. In the presence of NADH, using diaphorase as a catalyst, the MTT chromogene (a tetrazolium salt) is formed to a colored formazan. The colored formazan is quantified photometrically with a two-wavelength method (660 and 840 nm).
This unit is a perfect choice for Clinics that are looking for lab accurate results. Standard Glucose meters can be +/- 20% inaccurate. This is the ONLY CLIA waived analyzer on the market.
